Meso Scale Discovery assay is a proprietary technology to detect target analytes in complex biological matrices. This technology combines electrochemiluminescence detection and multi-array technology to deliver rapid and highly dense biological information through miniaturization and parallel processing of study samples. The Meso Scale Discovery multiplex assay includes diverse singleplex and multiplex kits, including MSD multiplex ELISA and MSD cytokine multiplex assays and a range of reagents and plates for assay development.
ELISA development is a unique approach for multiplexing immunoassays to quantify proteins and other biomarkers in biological samples. With an option of over 400 different assay kits along with custom assay development, Meso Scale Discovery assay have helped analytical lab testing services make precise and accurate determinations of proteins, cytokines, and biomarker levels in different matrices. These features have made several major biotech and pharmaceutical companies adopt Meso Scale Discovery multiplex assays for numerous clinical and biomedical research. The current article explores the benefits of Meso Scale Discovery assays in improving research efficiency.
How does the Meso Scale Discovery assay help improve research efficiency?
Compared to traditional ELISA assay, MSD has a broader dynamic range from 0.1 to 10 picogram per ml and higher sensitivity with a lower limit of detection between 0.1 to 1.4 picogram per ml. Moreover, MSD assays require a very low volume of study samples ranging from 1 to 25 microlitres, further saving precious biological fluids. Besides, MSD assays have minimal matrix effect and help in the simultaneous detection of analytes with high precision. MSD protocols are simpler, with reduced washing steps and rapid read times.
MSD is a highly versatile technology. The carbon electrode at the MSD microplate surface can be coated with different capture materials such as antibodies, carbohydrates, polysaccharides, peptides, proteins, and cell lysates. These diverse capture materials have benefited in several bioanalyses, including biologic testing, biomarker assay, immunogenicity testing, potency bioassay, identity bioassays, and PK/TK analysis. Besides, due to simplified workflows and rapid quantitative results, MSD assays are increasingly employed instead of traditional Western blotting techniques.
Besides, MSD assays are standardized against numerous biological samples such as cell lysates, serum, plasma, blood tissue homogenates, and cell culture supernatant. All these sample types make the MSD platform vital for the biopharmaceutical and drug development industry. Moreover, MSD provides custom fit-for-purpose and ready-to-use prevalidated singleplex and multiplex configurations. This panel configuration includes different sample matrices from humans, primates, canines, mice, and rats.
Multiplexing capacities are one of the benefits of MSD assays. MSD assays come in both 96 and 384-well plate formats. Besides, each well has up to 10 spots to capture antibodies. Hence each well can detect ten different analytes within a single reaction, exponentially increasing productivity and enhancing efficiency.
Understanding the presence and abundance of specific proteins is one of the most fundamental needs in preclinical and clinical research. This requirement is critical irrespective of whether you are profiling cytokines, tracking biomarkers, or evaluating ADA formation. Meso Scale Discovery assays fulfill all bioanalytical needs making them an integral component of analytical lab testing services.